Human indoleamine-2,3-dioxygenase (IDO) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-446

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: IDO

Size:

Human indoleamine-2,3-dioxygenase (IDO) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human indoleamine-2,3-dioxygenase (IDO) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IDO in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.145 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to IDO. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody specific to IDO. Next, avidin-HRP is introduced to each well and incubated. After the addition of TMB substrate, color development occurs only in wells containing the IDO sandwich complex. The enzyme-substrate reaction is terminated by adding a sulphuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of IDO in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: IDO is a key rate-limiting enzyme responsible for catalyzing the breakdown of the essential amino acid tryptophan. This degradation process constitutes the first step in the kynurenine pathway. IDO plays a significant role in various physiological and pathological processes, including immune regulation. Given its role in modulating inflammatory responses, it may contribute to the development and progression of obesity.

Synonyms: CD107B; INDO; Indoleamine-pyrrole 2,3 dioxygenase

Applications: Human indoleamine-2,3-dioxygenase (IDO) ELISA kit is used to quantify IDO in serum, plasma, and tissue homogenates, cell lysates, and other biological fluids of humans, providing data to support research in a wide range of areas, including enzyme & kinase, infection immunity, obesity, and others.

Research Area: Enzyme & kinase; Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.145 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out