Human hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL)

Cat. No.: 0126WXX-421

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: LIPE

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Human hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL)

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Product Overview

Description: Human hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in human samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The LIPE ELISA kit utilizes a competitive enzyme immunoassay technique. A monoclonal anti-LIPE antibody and an LIPE-HRP conjugate are incubated with the sample and buffer in a pre-coated plate for one hour. During this period, endogenous LIPE competes with the LIPE-HRP conjugate for a limited number of antibody binding sites. After incubation, the wells are washed five times. An HRP substrate is then added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm, where the color intensity is inversely proportional to the LIPE concentration. The LIPE concentration in each sample is determined by interpolating the O.D. values against a standard curve.

Background: LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.

Synonyms: HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Human hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL) is used to quantify LIPE in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of human, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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