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Human hormone sensitive lipase (LIPE) ELISA kit (0.313-20 ng/mL)
Cat. No.:
0126WXX-420
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
LIPE
Size:
Add to basket
Product Overview
Description:
Human hormone sensitive lipase (LIPE) ELISA kit (0.313-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in human samples with a range of 0.313-20 ng/mL and a minimum detectable dose (sensitivity) of 0.188 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
This kit employs the sandwich-ELISA method. The microplate is pre-coated with an antibody specific to LIPE. Standards or samples are added to the wells and combined with the specific capture antibody. Subsequently, a biotinylated detection antibody and avidin-HRP conjugate are added successively and incubated. After washing away free components, a substrate solution is added, appearing blue in positive wells and turning yellow after the addition of sulphuric acid. The OD is measured at 450 nm ± 2 nm. The OD value is directly proportional to the LIPE concentration, which is calculated by comparing the sample absorbance to the standard curve.
Background:
LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.
Synonyms:
HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79
EC NO.:
3.1.1.79
Applications:
Human hormone sensitive lipase (LIPE) ELISA kit (0.313-20 ng/mL) is used to quantify LIPE in serum, plasma, and biological fluids of humans, providing data to support research in a wide range of areas, including metabolism, obesity, and others.
Research Area:
Metabolism; Obesity
Specification
Sample Type:
Serum; Plasma; Biological fluids
Detection Range:
0.313-20 ng/mL
Sensitivity:
0.188 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Recovery:
91-99%
Storage:
Store at 4°C.
