Human glutathione peroxidase 1 (GPX1) ELISA kit (39.062-2500 pg/mL)

Cat. No.: 0126WXX-258

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: GPX1

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Human glutathione peroxidase 1 (GPX1) ELISA kit (39.062-2500 pg/mL)

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Product Overview

Description: Human glutathione peroxidase 1 (GPX1) ELISA kit (39.062-2500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GPX1 in human samples with a range of 39.062-2500 pg/mL and a minimum detectable dose (sensitivity) of 15 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on GPX1 antibody-GPX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX1 antigen targets in samples.

Background: GPX1 is an antioxidant enzyme widely distributed in the cytoplasm, mitochondria, and nucleus. It exists as a homotetramer and primarily functions to protect cells and enzymes from oxidative damage. GPX1 maintains intracellular redox homeostasis by catalyzing the reduction of hydrogen peroxide and lipid peroxides. It plays a crucial role in multiple biological processes, including apoptosis, lipid metabolism, inflammatory responses, and the response to oxidative stress. Oxidative stress is considered one of the causes for the development of obesity-related metabolic disorders. GPx-1 is a potential regulator of obesity.

Synonyms: GSHPX1; Cellular glutathione peroxidase

Applications: Human glutathione peroxidase 1 (GPX1) ELISA kit (39.062-2500 pg/mL) is used to quantify GPX1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of human, providing data to support research in a wide range of areas, including endocrinology, inflammation, oxidative stress, metabolism, obesity, and others.

Research Area: Endocrinology; Inflammation; Oxidative stress; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 39.062-2500 pg/mL

Sensitivity: 15 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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