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Human fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL, 4.37 pg/mL)
Cat. No.:
OB0625WXX-884
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
FGF21
Size:
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Product Overview
Description:
Human fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL, 4.37 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FGF21 in human with a range of 15.6-1000 pg/mL and a specificity of 4.37 pg/mL.
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an antibody specific for FGF2. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated FGF2-specific antibody preparation. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. Upon addition of the TMB substrate solution, only the wells containing FGF2, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of FGF2 in the sample can then be determined by comparing the OD of the sample to a standard curve.
Background:
FGF21 is a member of the FGF family and is the primary endogenous agonist for the FGF21 receptor. It is involved in various physiological processes and is associated with obesity. Studies have found that treatment with FGF21 analogues can normalize glucose utilization in obese mice and have shown some anti-obesity effects.
Synonyms:
FGF-21; Fgf21
Applications:
Human fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL, 4.37 pg/mL) is used to quantify FGF21 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of human, providing data to support a wide range of studies.
Research Area:
Cytokine; Metabolism; Obesity research
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
15.6-1000 pg/mL
Sensitivity:
4.37 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).
