Human family with sequence similarity 3, member B (FAM3B) ELISA kit

Cat. No.: OB0625WXX-817

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: FAM3B

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Human family with sequence similarity 3, member B (FAM3B) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Human family with sequence similarity 3, member B (FAM3B) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAM3B in human with a range of 0.625-40 ng/mL and a specificity of 0.255 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an antibody specific for FAM3B. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for FAM3B. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of the TMB substrate solution, only the wells containing FAM3B, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of FAM3B in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: FAM3B is a secreted protein belonging to the FAM3 family that is thought to function as a cytokine or signaling molecule involved in glucose metabolism and pancreatic beta cell function.

Synonyms: ORF9; PANDER; PRED44; C21orf11; C21orf76; Cytokine-like protein 2-21; Pancreatic-derived factor

Applications: Human family with sequence similarity 3, member B (FAM3B) ELISA kit is used to quantify FAM3B in serum, plasma, and other biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Carbohydrate metabolism