Human CYP21A2 ELISA kit (31.25-2000 pg/mL)

Cat. No.: OB0625WXX-563

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: CYP21A2

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Human CYP21A2 ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Human CYP21A2 ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CYP21A2 in human with a range of 31.25-2000 pg/mL and a specificity of 18.75 pg/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific for CYP21A2. Standards or samples are added to the appropriate micro ELISA plate wells and bound to the specific antibody. A biotinylated detection antibody specific for CYP21A2 and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing CYP21A2, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of CYP21A2. The concentration of CYP21A2 in the sample can be calculated by comparing the OD value of the sample with the standard curve.

Background: CYP21A2 belongs to the Cytochrome P450 family and is essential for the adrenal synthesis of mineralocorticoids and glucocorticoids. Deficiency of this enzyme is associated with certain diseases.

Synonyms: Cytochrome P450 family 21 subfamily A polypeptide 2; Steroid 21-hydroxylase; 21-OHase; EC 1.14.14.16

EC NO.: 1.14.14.16

Applications: Human CYP21A2 ELISA kit (31.25-2000 pg/mL) is used to quantify CYP21A2 in serum, plasma, and biological fluid samples of humans, providing data to support a wide range of studies.

Research Area: Enzyme and Kinase; Metabolic pathway; Endocrinology; Hormone metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 31.25-2000 pg/mL

Sensitivity: 18.75 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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