Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL)

Cat. No.: OB0525WXX-252

Assay Type: Quantitative sandwich ELISA

Target Species: Chicken

Assay Target: ADP

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Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL)

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Product Overview

Description: Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL) is a ready-to-use ELISA kit for analyzing chicken ADP levels with a detection range of 0.625-40 ng/mL and a sensitivity of 0.292 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADP. Avidin conjugated to HRP is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only the wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in chicken serum, plasma, and other biological fluid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.625-40 ng/mL

Sensitivity: 0.292 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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