Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.16-10 ng/mL)

Cat. No.: OB0625WXX-351

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: CB1

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Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.16-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.16-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CB1 in human with a range of 0.16-10 ng/mL and a specificity of 0.1 ng/mL.

Assay Principle: The microtiter ELISA plates in this kit are pre-coated with an antibody specific to Human CB1. Standards or samples are added into the wells of the microtiter ELISA plate to bind to the specific antibody. A biotinylated detection antibody specific for human CB1 and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microtiter plate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing human CB1, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of the termination solution, the enzyme-substrate reaction terminates, and the color turns yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of human CB1. The concentration of human CB1 in the sample is calculated by comparing the OD value of the sample with the standard curve.

Background: CB1 is a member of the cannabinoid receptor family and the G protein-coupled receptor (GPCR) superfamily. It is expressed at the highest levels in different brain regions, and is also present at lower levels in most other cell/tissue types, including adipose tissue. It is thought to activate multiple signaling pathways, regulating energy metabolism and cell survival/death. CB1 has become a drug target for treating obesity. CB1 inhibitors may be potential anti-obesity drugs.

Synonyms: CNR1; Central cannabinoid receptor; Brain-type cannabinoid receptor

Applications: Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.16-10 ng/mL) is used to quantify CB1 in serum, plasma, and other biological fluid samples of humans, providing data to support a wide range of studies.

Research Area: Lipolysis; Glucose uptake; Energy metabolism; Cell growth; Signaling pathway; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.16-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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