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Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.055 ng/mL)
Cat. No.:
OB0625WXX-346
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
CB1
Size:
Add to basket
Product Overview
Description:
Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.055 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CB1 in human with a range of 0.156-10 ng/mL and a specificity of 0.055 ng/mL.
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an antibody specific to brain CB1. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific to brain CB1. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of the TMB substrate solution, only wells containing brain CB1, Biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of brain CB1 in the sample.
Background:
CB1 is a member of the cannabinoid receptor family and the G protein-coupled receptor (GPCR) superfamily. It is expressed at the highest levels in different brain regions, and is also present at lower levels in most other cell/tissue types, including adipose tissue. It is thought to activate multiple signaling pathways, regulating energy metabolism and cell survival/death. CB1 has become a drug target for treating obesity. CB1 inhibitors may be potential anti-obesity drugs.
Synonyms:
CNR1; Central cannabinoid receptor; Brain-type cannabinoid receptor
Applications:
Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.055 ng/mL) is used to quantify CB1 in tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of human, providing data to support a wide range of studies.
Research Area:
Lipolysis; Glucose uptake; Energy metabolism; Cell growth; Signaling pathway; Obesity research
Specification
Sample Type:
Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.055 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
