Human beta 3 adrenergic receptor (ADRB3) ELISA kit

Cat. No.: OB0525WXX-337

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: ADRB3

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Human beta 3 adrenergic receptor (ADRB3) ELISA kit

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Product Overview

Description: Human beta 3 adrenergic receptor (ADRB3) ELISA kit is a ready-to-use ELISA kit for analyzing human ADRB3 levels with a sensitivity of 1.0 pg/mL.

Assay Principle: The kit is based on a competitive enzyme immunoassay technique using a polyclonal anti-ADRB3 antibody and an ADRB3-HRP conjugate. The assay sample and buffer are incubated with the ADRB3-HRP conjugate on a pre-coated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with HRP enzyme substrate. The product of the enzyme reaction with the substrate forms a blue colored complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of ADRB3 because the ADRB3 in the sample competes with the ADRB3-HRP conjugate for the binding site of the anti-ADRB3 antibody. Since there is a finite number of binding sites, as more sites are occupied by ADRB3 in the sample, there are fewer sites left to bind the ADRB3-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The ADRB3 concentration in each sample is derived from this standard curve.

Background: ADRB3, the β3-adrenergic receptor, regulates lipolysis, thermogenesis in brown adipose tissue, and vasodilation. β3-adrenergic receptor agonists have been found to have significant anti-obesity and anti-diabetic effects. ADRB3 is an attractive target in anti-obesity research.

Synonyms: β3AR; β3-AR; Beta-3 adrenoceptor

Applications: Human beta 3 adrenergic receptor (ADRB3) ELISA kit is designed for the in vitro quantitative analysis of ADRB3 levels in human serum, plasma, cell culture supernatants, body fluid, and tissue homogenate samples.

Research Area: Thermogenesis; Lipolysis; Signaling pathways; Metabolism; Adipocyte differentiation; Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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