Human adipose triglyceride lipase (ATGL) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1405

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ATGL

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Human adipose triglyceride lipase (ATGL) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Human adipose triglyceride lipase (ATGL) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ATGL in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.118 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The methodology employed in this kit is a sandwich enzyme immunoassay. The provided microtiter plate is pre-immobilized with a capture antibody specific to ATGL. Standards or samples are introduced into the designated microtiter wells along with a biotin-conjugated detection antibody specific for ATGL. Subsequently, avidin-conjugated HRP is added to each microplate well and incubated. Upon the addition of TMB substrate solution, color development occurs exclusively in wells containing the ATGL-biotin-avidin sandwich complex. The enzymatic reaction is terminated with a sulphuric acid solution, and the color change is recorded spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of ATGL in the samples is then determined by comparing the optical density (O.D.) of the specimens to the established standard curve.

Background: ATGL, also known as PNPLA2, is the rate-limiting enzyme in the intracellular lipolysis pathway and a key member of the Triglyceride Lipase (TGL) family. ATGL primarily catalyzes the first step in the hydrolysis of triglycerides (TG) stored in cellular lipid droplets into diglycerides and free fatty acids (FFA). In obesity research, ATGL occupies a central position as it directly governs energy storage and mobilization. Dysregulation of ATGL activity leads to ectopic TG accumulation in non-adipose tissues such as the liver and muscle, triggering lipotoxicity and associated pathological responses. Furthermore, ATGL activity influences FFA release, thereby affecting systemic energy balance and adipose tissue inflammation. Consequently, ATGLs represent key targets for understanding lipolysis dysregulation and obesity-related metabolic complications.

Synonyms: PEDF-R; TTS2; Desnutrin; Pigment epithelium-derived factor; Transport-secretion protein 2; Calcium-independent phospholipase A2; PNPLA2; Patatin like phospholipase domain containing protein 2

Applications: Human adipose triglyceride lipase (ATGL) ELISA kit (0.312-20 ng/mL) is used to quantify ATGL in tissue homogenates, cell lysates, and other biological fluids of humans, providing data to support research in a wide range of areas, including lipid metabolism, energy balance and thermogenesis, and others.

Research Area: Lipid metabolism; Energy balance and thermogenesis