Human A kinase anchor protein 1 (AKAP1) ELISA kit (0.625-40 ng/mL)

Cat. No.: OB0625WXX-037

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: AKAP1

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Human A kinase anchor protein 1 (AKAP1) ELISA kit (0.625-40 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human A kinase anchor protein 1 (AKAP1) ELISA kit (0.625-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of AKAP1 in human with a range of 0.625-40 ng/mL and a specificity of 0.375 ng/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific to AKAP1. Standards or samples are added to the corresponding wells of the microtiter plate and bound to the specific antibody. The biotinylated detection antibody specific for AKAP1 and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. Free components are washed away. Substrate solution is added to each well. Only wells containing AKAP1, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon the addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of AKAP1. The concentration of AKAP1 in the sample is calculated by comparing the OD value of the sample with the standard curve.

Background: AKAP1 is a group of structurally diverse proteins, containing unique lipid or protein binding domains. It has the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete 1 intracellular locations.

Synonyms: AKAP; A kinase (PRKA) anchor protein 1; Protein kinase A anchoring protein 1; PRKA1

Applications: Human A kinase anchor protein 1 (AKAP1) ELISA kit (0.625-40 ng/mL) is used to quantify AKAP1 in serum, plasma, and biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Signal transduction; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.625-40 ng/mL

Sensitivity: 0.375 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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