Guinea pig ghrelin (GHRL) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-164

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: GHRL

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Guinea pig ghrelin (GHRL) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

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Product Overview

Description: Guinea pig ghrelin (GHRL) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GHRL in guinea pig samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a sandwich ELISA technique for guinea pig GHRL. The plate is pre-coated with a monoclonal capture antibody. After adding samples and washing with PBS or TBS, a biotin-labeled polyclonal detection antibody is added to form a sandwich. An avidin-peroxidase conjugate is then introduced. After a final wash, TMB substrate is added, shifting the solution color to blue and then yellow upon acidification. The color intensity is directly proportional to the sample GHRL concentration.

Background: Ghrelin is a peptide hormone primarily secreted by the stomach, exerting its effects by binding to the growth hormone secretagogue receptor type 1 (GHSR). It plays multiple roles in the body, but its most well-known function is regulating energy balance and appetite. Research indicates that ghrelin levels and their regulation of appetite may be abnormal in obese individuals. It is considered a key target for developing anti-obesity drugs.

Synonyms: MTLRP; Preproghrelin; Growth hormone secretagogue receptor ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Applications: Guinea pig ghrelin (GHRL) ELISA kit is used to quantify GHRL in guinea pig serum, plasma, cell culture supernatant, and organizations of guinea pig, providing data to support research in a wide range of areas, including signal transduction, endocrinology, gastroenterology, metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Gastroenterology; Metabolism; Obesity

Specification

Sample Type: Guinea pig serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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