Goat insulin (INS) ELISA kit (0.1-40 mIU/L)

Cat. No.: 0126WXX-560

Assay Type: Quantitative sandwich ELISA

Target Species: Goat

Assay Target: INS

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Goat insulin (INS) ELISA kit (0.1-40 mIU/L)

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Product Overview

Description: Goat insulin (INS) ELISA kit (0.1-40 mIU/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in goat samples with a range of 0.1-40 mIU/L and a minimum detectable dose (sensitivity) of 0.058 mIU/L. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes a sandwich immunoassay format featuring a microplate pre-coated with a goat INS capture antibody. INS present in the sample binds to the immobilized antibody, followed by the addition of a biotinylated goat INS detection antibody and streptavidin-HRP. After a wash step to remove unbound reagents, a substrate solution is added, initiating color development in direct proportion to the amount of INS. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Goat insulin (INS) ELISA kit (0.1-40 mIU/L) is used to quantify INS in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of goat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.1-40 mIU/L

Sensitivity: 0.058 mIU/L

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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