Goat hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL)

Cat. No.: 0126WXX-415

Assay Type: Quantitative competitive ELISA

Target Species: Goat

Assay Target: LIPE

Size:

Goat hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Goat hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in goat samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The LIPE ELISA kit utilizes a quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for LIPE is pre-coated onto the microtiter plate. Standards or samples are added to the wells, where any LIPE present binds to the immobilized antibody. To quantify the analyte, an HRP-conjugated polyclonal antibody specific for LIPE is added to form a "sandwich" complex. Following incubation and thorough washing, substrate solutions are introduced. The enzyme-substrate reaction produces a color change in wells containing the LIPE complex. The reaction is terminated with sulphuric acid, and the absorbance is measured at 450 nm. LIPE concentrations are determined by interpolating the O.D. values against a standard curve.

Background: LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.

Synonyms: HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Goat hormone sensitive lipase (LIPE) ELISA kit (0.5-10 ng/mL) is used to quantify LIPE in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of goat, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out