Dog growth hormone (GH) ELISA kit (123.5-10000 pg/mL)

Cat. No.: 0126WXX-105

Assay Type: Quantitative competitive ELISA

Target Species: Dog

Assay Target: GH

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Dog growth hormone (GH) ELISA kit (123.5-10000 pg/mL)

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Product Overview

Description: Dog growth hormone (GH) ELISA kit (123.5-10000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in dog samples with a range of 123.5-10000 pg/mL and a minimum detectable dose (sensitivity) of 52.9 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GH is pre-coated onto the microplate. A competitive reaction occurs between biotin-labeled GH and unlabeled GH (standards or samples) for the limited binding sites on the immobilized antibody. After washing away unbound conjugates, avidin-HRP is added and incubated. The amount of bound HRP conjugate and the resulting color intensity are inversely proportional to the concentration of GH in the sample.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Dog growth hormone (GH) ELISA kit (123.5-10000 pg/mL) is used to quantify GH in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of dog, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 123.5-10000 pg/mL

Sensitivity: 52.9 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 88-101%

Storage: Store at 2-8°C.

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