Rat fibroblast growth factor 21 (FGF21) ELISA kit (1.56-100 pg/mL)

Cat. No.: 0126WXX-08

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: FGF21

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Rat fibroblast growth factor 21 (FGF21) ELISA kit (1.56-100 pg/mL)

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Product Overview

Description: Rat fibroblast growth factor 21 (FGF21) ELISA kit (1.56-100 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FGF21 in rat samples with a range of 1.56-100 pg/mL and a minimum detectable dose (sensitivity) of 0.69 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-immobilized with an antibody specific to FGF21. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody. Next, avidin-HRP is added and incubated. After the addition of TMB substrate, color develops only in wells containing the target complex. The enzymatic reaction is terminated with sulphuric acid, and the absorbance is measured at 450 nm ± 10 nm. The final concentration is determined by comparing the sample O.D. to the standard curve.

Background: FGF21 is an endocrine hormone secreted by multiple tissues, particularly the liver. It belongs to the fibroblast growth factor family but functions differently from traditional growth factors. FGF21 is primarily induced during stress or fasting states, acting as a crucial metabolic regulator that influences the body's energy balance, glucose homeostasis, and fatty acid metabolism. FGF21 plays a pivotal role in the onset, progression, and treatment of obesity. Its analogues have emerged as novel therapeutic agents and are being investigated for their potential in anti-obesity interventions.

Synonyms: Fgf21; FGF-21

Applications: Rat fibroblast growth factor 21 (FGF21) ELISA kit (1.56-100 pg/mL) is used to quantify FGF21 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of rat, providing data to support research in a wide range of areas, including cytokine, metabolism, obesity, and others.

Research Area: Cytokine; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 1.56-100 pg/mL

Sensitivity: 0.69 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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