Chicken cholecystokinin (CCK) ELISA kit

Cat. No.: OB0625WXX-286

Assay Type: Quantitative sandwich ELISA

Target Species: Chicken

Assay Target: CCK

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Product Overview

Description: Chicken cholecystokinin (CCK) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CCK in chicken with a range of 15.625-1000 pg/mL and a specificity of 9.375 pg/mL.

Assay Principle: The anti-CCK antibody is pre-coated onto a 96-well plate. The biotin-conjugated anti-CCK antibody is then used as the detection antibody. Standards, assay samples, and biotin-conjugated detection antibody are then added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to observe the HRP enzymatic reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The concentration of yellow color is proportional to the amount of CCK sample captured in the plate. The concentration of CCK is calculated by reading the absorbance at 450 nm with a microplate reader.

Background: CCK is a peptide hormone produced in the small intestine that primarily stimulates gallbladder contraction and pancreatic enzyme release, and also plays a role in satiety.

Synonyms: CCK-PZ

Applications: Chicken cholecystokinin (CCK) ELISA kit is used to quantify CCK in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of chicken, providing data to support a wide range of studies.

Research Area: Tumor immunity;Endocrinology;Neuro science;Hepatology;Hormone metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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