Canine leptin (LEP) ELISA kit (156-10000 ng/mL)

Cat. No.: 0126WXX-790

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: LEP

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Canine leptin (LEP) ELISA kit (156-10000 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine leptin (LEP) ELISA kit (156-10000 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in canine samples with a range of 156-10000 ng/mL and a minimum detectable dose (sensitivity) of 46.8 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on LEP antibody-LEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEP antigen targets in samples.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Canine leptin (LEP) ELISA kit (156-10000 ng/mL) is used to quantify LEP in serum, plasma, and other biological fluids of canine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 156-10000 ng/mL

Sensitivity: 46.8 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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