Canine fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL)

Cat. No.: OB0625WXX-824

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: FAS

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Canine fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL)

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Product Overview

Description: Canine fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in canine with a range of 50-1000 μg/mL and a specificity of 1.0 μg/mL.

Assay Principle: The assay samples and buffer are incubated with the FAS-HRP conjugate in a pre-coated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with substrate for the HRP enzyme. The product of the reaction of the enzyme with the substrate forms a blue colored complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of FAS because the FAS in the sample competes with the FAS-HRP conjugate for the binding site of the anti-FAS antibody. Since there are a limited number of binding sites, as more sites are occupied by FAS in the sample, fewer sites remain to bind the FAS-HRP conjugate. A standard curve is plotted to relate the color intensity (O.D.) to the concentration of the standard. The concentration of FAS in each sample is derived from this standard curve.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Canine fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL) is used to quantify FAS in serum, plasma, cell culture supernatants, body fluid, tissue homogenate samples of canine, providing data to support a wide range of studies.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 50-1000 μg/mL

Sensitivity: 1.0 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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