Bovine lipopolysaccharide binding protein (LBP) ELISA kit (15.6-1000 ng/mL)

Cat. No.: 0126WXX-753

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: LBP

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Bovine lipopolysaccharide binding protein (LBP) ELISA kit (15.6-1000 ng/mL)

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Product Overview

Description: Bovine lipopolysaccharide binding protein (LBP) ELISA kit (15.6-1000 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in porcine samples with a range of 15.6-1000 ng/mL and a minimum detectable dose (sensitivity) of <3.9 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. A microplate is pre-coated with an antibody specific to LBP. Standards and samples are added to the wells, where any LBP present is immobilized by the capture antibody. After washing away unbound substances, a biotin-conjugated detection antibody for LBP is added. Following a subsequent wash, avidin-conjugated HRP is introduced. After a final wash to remove any unbound reagents, a substrate solution is added. Color develops in direct proportion to the amount of LBP bound, which is then measured spectrophotometrically after stopping the reaction.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Bovine lipopolysaccharide binding protein (LBP) ELISA kit (15.6-1000 ng/mL) is used to quantify LBP in serum, plasma, and tissue homogenates of bovine, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 15.6-1000 ng/mL

Sensitivity: <3.9 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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