Bovine leptin (LEP) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-788

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: LEP

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Bovine leptin (LEP) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Bovine leptin (LEP) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in porcine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.060 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The test principle applied in this kit is a sandwich enzyme immunoassay. The microtiter plate provided has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate wells along with a biotin-conjugated antibody specific to LEP. Subsequently, avidin conjugated to HRP is added to each well and incubated to form an immune complex. Following the addition of TMB substrate solution, a colorimetric change occurs only in wells containing the LEP-antibody-avidin complex. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the resulting color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of LEP in the samples is then determined by comparing the optical density (O.D.) of the samples to a generated standard curve.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Bovine leptin (LEP) ELISA kit (0.156-10 ng/mL) is used to quantify LEP in serum, plasma, tissue homogenates, and cell lysates, cell culture supernatants, and other biological fluids of bovine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.060 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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