Bovine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-694

Assay Type: Quantitative competitive ELISA

Target Species: Bovine

Assay Target: IRS-1

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Bovine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

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Product Overview

Description: Bovine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IRS-1 in porcine samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This IRS1 ELISA kit employs a competitive enzyme immunoassay technique. It utilizes a polyclonal anti-IRS1 antibody and an IRS1-HRP conjugate. Samples and assay buffers are co-incubated with the IRS1-HRP conjugate in a pre-coated microplate for one hour. During this process, endogenous IRS1 from the sample competes with the HRP-labeled conjugate for a limited number of antibody binding sites. After incubation, the wells are decanted and washed five times. An HRP-specific substrate is then added to form a blue complex, which turns yellow upon the addition of a stop solution. The resulting color intensity, measured at 450 nm, is inversely proportional to the IRS1 concentration. Sample concentrations are determined by interpolating the optical density (O.D.) against the standard curve.

Background: IRS-1 is a key adaptor protein that plays a central role in insulin signaling. When insulin binds to its receptor on the cell surface, the receptor is activated and phosphorylates tyrosine residues on IRS-1. This process transforms IRS-1 into a signaling “relay station,” enabling it to bind with multiple downstream signaling proteins such as phosphoinositide 3-kinase (PI3K). IRS-1 transmits insulin signals from the cell membrane into the cell interior, thereby initiating a series of vital biological responses, including promoting glucose transport and glycogen synthesis. Studying the function and defects of IRS-1 is crucial for understanding the pathological processes underlying obesity.

Synonyms: IRS1

Applications: Bovine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is used to quantify IRS-1 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of bovine, providing data to support research in a wide range of areas, including endocrinology, obesity, etc.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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