Porcine apolipoprotein C-III (apo-CIII) ELISA kit (1.0 ng/mL)

Cat. No.: OB0625WXX-162

Assay Type: Quantitative competitive ELISA

Target Species: Porcine

Assay Target: apo-CIII

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Porcine apolipoprotein C-III (apo-CIII) ELISA kit (1.0 ng/mL)

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Product Overview

Description: Porcine apolipoprotein C-III (apo-CIII) ELISA kit (1.0 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of apo-CIII in porcine with a specificity of 1.0 ng/mL. The kit is highly specific and easy to use.

Assay Principle: The assay samples and buffer are incubated with Apo-CIII-HRP conjugate in pre-coated plates for one hour. At the end of the incubation period, the wells are poured out and washed five times. The wells are then incubated with the substrate for the HRP enzyme. The product of the reaction of the enzyme with the substrate forms a blue colored complex. Finally, the reaction is stopped by the addition of a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of Apo-CIII because Apo-CIII and Apo-CIII-HRP conjugates in the sample compete for the binding site of the anti-Apo-CIII antibody. Since there are a limited number of binding sites, as more sites are occupied by Apo-CIII in the sample, there are fewer sites left to bind the Apo-CIII-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The Apo-CIII concentration in each sample is derived from this standard curve.

Background: Apolipoprotein C-III is secreted by the liver and small intestine. It is a very low-density lipoprotein (VLDL) protein that inhibits lipoprotein lipase and hepatic lipase. Studies have found that it plays a key role in the metabolism of triglyceride-rich lipoproteins (TRLs).

Synonyms: ApoC-III ; ApoCIII; Apo C-III; Apo C III; Apolipoprotein CIII; APOC3

Applications: Porcine apolipoprotein C-III (apo-CIII) ELISA kit (1.0 ng/mL) is an important tool for the quantitative detection of apo-CIII in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of porcine.

Research Area: Lipid metabolism; Fatty acid biosynthesis; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture superna tants; Body fluid; Tissue homogenate

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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