Bovine insulin (INS) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-529

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: INS

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Bovine insulin (INS) ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine insulin (INS) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in porcine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.061 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a sandwich enzyme immunoassay where a microtiter plate is pre-coated with an antibody specific to INS. Samples or standards are added along with a biotin-conjugated detection antibody and Avidin-HRP to form an immune complex. Following the addition of TMB substrate, a colorimetric change occurs that is terminated by sulphuric acid; the resulting optical density (O.D.) at 450nm is used to determine the INS concentration via a standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Bovine insulin (INS) ELISA kit (0.156-10 ng/mL) is used to quantify INS in bovine serum, plasma, and other biological fluids of bovine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Bovine serum; Plasma; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.061 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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