Bovine glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-301

Assay Type: Quantitative competitive ELISA

Target Species: Bovine

Assay Target: GSH-Px

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Bovine glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine glutathione peroxidase (GSH-Px) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GSH-Px in bovine samples with a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This GSH-Px ELISA kit utilizes a competitive enzyme immunoassay technique featuring a polyclonal anti-GSH-Px antibody and a GSH-Px-HRP conjugate. Samples and assay buffer are incubated with the GSH-Px-HRP conjugate in a pre-coated microplate for one hour. During this process, endogenous GSH-Px from the sample competes with the GSH-Px-HRP conjugate for a limited number of antibody binding sites. After the incubation period, the wells are washed five times. An HRP substrate is then added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm, where the color intensity is inversely proportional to the GSH-Px concentration. A standard curve is generated to relate the O.D. to standard concentrations, from which the GSH-Px level in each sample is interpolated.

Background: Glutathione peroxidase is a family of antioxidant enzymes widely distributed throughout the human body. Their core function is to protect cells from oxidative damage. Obesity itself is considered a chronic low-grade inflammatory state, often accompanied by significant oxidative stress. Glutathione peroxidase is a potential regulator of obesity.

Synonyms: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P; GPX3

Applications: Bovine glutathione peroxidase (GSH-Px) ELISA kit is used to quantify GSH-Px in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of bovine, providing data to support research in a wide range of areas, including oxidative stress, metabolism, obesity, and others.

Research Area: Oxidative stress; Metabolism; Obesity