Bovine cholecystokinin A receptor (CCKAR) ELISA kit

Cat. No.: OB0625WXX-288

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: CCKAR

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Bovine cholecystokinin A receptor (CCKAR) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine cholecystokinin A receptor (CCKAR) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CCKAR in bovine with a range of 0.312-20 ng/mL and a specificity of 0.119 ng/mL.

Assay Principle: The microtiter plates in this kit are pre-coated with an antibody specific for CCKAR. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for CCKAR. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only the wells containing CCKAR, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of CCKAR in the sample.

Background: Cholecystokinin A receptor is a G protein-coupled receptor that binds to non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. It plays a role in regulating satiety and the release of dopamine, among others.

Synonyms: CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin type 1 receptor

Applications: Bovine cholecystokinin A receptor (CCKAR) ELISA kit is used to quantify CCKAR in tissue homogenates and other biological fluid samples of bovine, providing data to support a wide range of studies.

Research Area: Feeding behavior; Obesity research

Specification

Sample Type: Tissue homogenates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.119 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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