Product Overview
Description:
AdipoUpX™ Human PPARG Ad5 Adenoviral Particle (Overexpression) is engineered for efficient overexpression of the human PPARG gene. It utilizes a highly potent promoter. This product serves as a powerful gene delivery tool for rapid functional validation of adipocyte differentiation, insulin sensitization, and lipid metabolism in obesity and diabetes research. Our customizable platform supports the flexible inclusion of reporter genes or resistance markers. Comprehensive QC is also performed, including titer, sterility, and mycoplasma testing.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
PPARG
Full Name:
Peroxisome proliferator activated receptor gamma
Gene Symbol:
GLM1; CIMT1; FPLD3; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
Summary:
The PPARG gene encodes the peroxisome proliferator-activated receptor gamma, a core member of the nuclear receptor superfamily. PPAR gamma forms heterodimers with the retinoid X receptor (RXR) and functions as a transcription factor to regulate gene expression. PPARγ serves as a master regulator of adipocyte differentiation and lipid metabolism, exhibiting high expression in adipose tissue. It is not only highly implicated in the pathogenesis of obesity and diabetes but is also recognized as a potential therapeutic target for atherosclerosis and cancer. Multiple splicing variants encode distinct PPARγ subtypes.
