Product Overview
Description:
AdipoUpX™ Human LEPR Ad5 Adenoviral Particle (Overexpression) enables the efficient transient overexpression of the human LEPR gene through a highly efficient promoter. LEPR is the key entry point for LEP signaling. This allows systematic investigation into leptin resistance and downstream JAK/STAT activation. The product thereby serves as a powerful tool for in vitro functional validation and signaling studies. Product quality is guaranteed by rigorous purification, titer validation, and comprehensive QC screening against contaminants.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
LEPR
Full Name:
Leptin receptor
Gene Symbol:
OBR; OB-R; CD295; LEP-R; LEPRD
Summary:
The protein encoded by the LEPR gene is a membrane-bound receptor belonging to the 130 family of cytokine receptors, which are known to stimulate gene transcription via the activation of cytosolic STAT proteins. This protein serves as the primary receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is centrally involved in the regulation of fat metabolism and a novel hematopoietic pathway required for normal lymphopoiesis. Mutations in this gene have been associated with both obesity and pituitary dysfunction. Furthermore, alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and the LEPROT gene share the same promoter and the first two exons, yet encode distinct proteins.
