Product Overview
Description:
AdipoUpX™ Human INS Ad5 Adenoviral Particle (Overexpression) utilizes a highly efficient promoter for the overexpression of the human INS (insulin) gene in mammalian cells. This makes it an ideal tool for metabolic signaling research and diabetes studies. It facilitates investigation into insulin biosynthesis and its systemic role in glucose homeostasis and anabolic lipid storage. Product integrity is ensured by comprehensive QC screening against contaminants.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
INS
Full Name:
Insulin
Gene Symbol:
IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
Summary:
The INS gene governs the production of Insulin, the core peptide hormone controlling carbohydrate and lipid metabolism. The gene product, Proinsulin, undergoes two precise post-translational cleavages to yield the mature, bioactive Insulin (A and B chains linked by disulfide bonds) and the C-peptide. The integrity of this hormone is vital, as its binding to the INSR triggers the systemic mechanism for glucose uptake. Genetic defects in INS can cause a spectrum of disorders, including permanent neonatal diabetes, different types of Maturity-Onset Diabetes of the Young (MODY) type 10, and conditions defined by excess immature hormone (hyperproinsulinemia).
