Product Overview
Description:
AdipoUpX™ Human IL13 Ad5 Adenoviral Particle (Overexpression) enables the efficient transient overexpression of the human IL13 gene through a highly efficient promoter. As an immunoregulatory cytokine, it modulates macrophage activity and protects insulin sensitivity by inhibiting inflammatory chemicals. This product serves as a powerful tool for investigating immune-metabolic cross-talk, with quality guaranteed by rigorous purification and comprehensive QC screening.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
IL13
Full Name:
Interleukin 13
Gene Symbol:
P600; IL-13
Summary:
IL13 encodes an immunomodulatory cytokine predominantly produced by activated Th2 lymphocytes. It regulates multiple stages of B-cell maturation and differentiation by enhancing CD23 and MHC class II expression and promoting IgE class switching. IL13 also suppresses macrophage activation, thereby limiting the production of pro-inflammatory cytokines and chemokines. While IL13 is a key driver of allergen-induced asthma through mechanisms independent of IgE and eosinophils, it also contributes to immune regulation within adipose tissue. Altered IL13 signaling has been implicated in obesity-associated inflammation. IL13 is part of a cytokine gene cluster on chromosome 5q, located in proximity to IL4.
