Product Overview
Description:
AdipoUpX™ Human HNF4A Ad5 Adenoviral Particle (Overexpression) is specifically designed for efficient transient overexpression of the human HNF4A gene. As a master transcription factor in the liver and pancreas, HNF4A regulates genes involved in lipid transport and glucose-stimulated insulin secretion. This tool accelerates functional validation of its role in MODY1 and metabolic health. High customization via reporter genes and comprehensive QC are integrated.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
HNF4A
Full Name:
Hepatocyte nuclear factor 4 alpha
Gene Symbol:
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha
Summary:
HNF4A encodes a nuclear transcription factor that binds DNA as a homodimer and regulates the expression of numerous genes involved in glucose and lipid metabolism. It acts upstream of other hepatic transcription factors, including HNF1A, and is essential for normal liver, intestinal, and pancreatic function. Mutations in HNF4A cause autosomal dominant monogenic diabetes and are associated with dysregulated energy metabolism, which may predispose affected individuals to obesity-related metabolic complications. Multiple isoforms arise through alternative splicing.
