AdipoUpX™ Human FTO Ad5 Adenoviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human FTO Ad5 Adenoviral Particle (Overexpression) is a ready-to-use Ad5 adenovirus vector particle. It has been fully validated for titer and purity. This product is specifically designed for the efficient transient overexpression of the human FTO gene. It enables rapid cellular validation of FTO's effects on nucleic acid demethylation, fat accumulation, and metabolic signaling pathways. This product is supported by flexible reporter gene selection and comprehensive quality control screening.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type5 (dE1/E3)

Promoter: CMV

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.

Sterility Test: This product was confirmed to be sterile upon testing.

Mycoplasma Test: This product has passed quality control, confirming that it is free of mycoplasma contamination.

Other QC: We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.

Storage: Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.

Stability: The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.

Shipping Condition: Our adenovirus viral products are shipped using dry ice.

Handling Notes: Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.

Target Profile

Gene Name: FTO

Full Name: Alpha-ketoglutarate-dependent dioxygenase

Gene Symbol: GDFD; IFEX9; ALKBH9; BMIQ14

Gene ID: 79068

RefSeq ID: NM_001080432.3, NP_001073901.1

Summary: The protein encoded by the FTO gene is a nuclear protein belonging to the AlkB-related non-heme iron and 2-oxoglutarate-dependent oxygenase superfamily. Other non-heme iron enzymes in this family are known to reverse DNA and RNA alkylation damage via oxidative demethylation. Studies in both mouse and human models indicate that this gene plays roles in the nervous and cardiovascular systems, and is significantly associated with body mass index (BMI), obesity risk, and type 2 diabetes mellitus.

AdipoUpX™ Human FTO Ad5 Adenoviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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