Rat proinsulin (PI) ELISA kit (1-300 pmol/L)

Cat. No.: 0126WXX-1283

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PI

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Rat proinsulin (PI) ELISA kit (1-300 pmol/L)

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Product Overview

Description: Rat proinsulin (PI) ELISA kit (1-300 pmol/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PI in rat samples with a range of 1-300 pmol/L and a minimum detectable dose (sensitivity) of 0.55 pmol/L. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rat PI antibody. The PI present in the sample binds to the immobilized capture antibodies. A biotinylated rat PI detection antibody is then added, followed by streptavidin-HRP. After incubation and washing away unbound components, a substrate solution is introduced. Color development occurs in proportion to the concentration of rat PI. The reaction is terminated with an acidic stop solution, and the absorbance is recorded at 450 nm.

Background: Proinsulin is the precursor molecule of insulin, synthesized in pancreatic β cells and subsequently processed into mature insulin and C-peptide. It plays a critical role in glucose homeostasis by serving as an essential intermediate in insulin biosynthesis and secretion. Under normal physiological conditions, proinsulin is efficiently converted to insulin; however, impaired processing or increased proinsulin secretion reflects β-cell dysfunction. Elevated circulating proinsulin levels are commonly observed in obesity and insulin resistance, making proinsulin a key indicator of metabolic stress and a contributing factor to the development of obesity-associated type 2 diabetes.

Synonyms: INS; Pro-Insulin; Preproinsulin

Applications: Rat proinsulin (PI) ELISA kit (1-300 pmol/L) is used to quantify PI in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of rats, providing data to support research in a wide range of areas, including endocrinology, hormone metabolism, obesity, and others.

Research Area: Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 1-300 pmol/L

Sensitivity: 0.55 pmol/L

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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