Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-40 ng/mL)

Cat. No.: 0126WXX-1494

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PPARα

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Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-40 ng/mL)

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Product Overview

Description: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in rat samples with a range of 0.5-40 ng/mL and a minimum detectable dose (sensitivity) of 0.22 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The plate is pre-coated with a rat PPARα antibody. PPARα present in the sample binds to the immobilized antibodies, followed by the binding of a biotinylated rat PPARα detection antibody and streptavidin-HRP. After incubation and a wash step, a substrate solution is added. Color develops in direct proportion to the amount of rat PPARα and is measured at 450 nm after the reaction is halted with an acidic stop solution.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-40 ng/mL) is used to quantify PPARα in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of rats, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.5-40 ng/mL

Sensitivity: 0.22 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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