Rat peptide YY (PYY) ELISA kit-Quantitative sandwich (15.625-1000 pg/mL)

Cat. No.: 0126WXX-1678

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PYY

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Rat peptide YY (PYY) ELISA kit-Quantitative sandwich (15.625-1000 pg/mL)

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Product Overview

Description: Rat peptide YY (PYY) ELISA kit-Quantitative sandwich (15.625-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in rat samples with a range of 15.625-1000 pg/mL and a minimum detectable dose (sensitivity) of 9.375 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA principle. The provided microplate is pre-coated with an antibody specific to rat PYY. Standards or samples are added to the wells to bind with the capture antibody. A biotinylated detection antibody and an avidin-HRP conjugate are then added successively and incubated. After washing away free components, a substrate solution is introduced. Wells containing the rat PYY complex will turn blue and then yellow upon the addition of the stop solution. The O.D., measured at 450 nm ± 2 nm, is directly proportional to the concentration of rat PYY.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Rat peptide YY (PYY) ELISA kit-Quantitative sandwich (15.625-1000 pg/mL) is used to quantify PYY in serum, plasma, and biological fluids of rats, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 86-105%

Storage: Store at 2-8°C.

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