Rat peptide YY (PYY) ELISA kit (7.8-500 pg/mL)

Cat. No.: 0126WXX-1675

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PYY

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Rat peptide YY (PYY) ELISA kit (7.8-500 pg/mL)

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Product Overview

Description: Rat peptide YY (PYY) ELISA kit (7.8-500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in rat samples with a range of 7.8-500 pg/mL and a minimum detectable dose (sensitivity) of 1.95 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a quantitative sandwich enzyme immunoassay technique. An antibody specific for PYY is pre-coated onto the microplate. Standards and samples are pipetted into the wells, where any PYY present is immobilized by the capture antibody. After washing away unbound substances, a biotin-conjugated detection antibody specific for PYY is added. Following a subsequent wash, Avidin-conjugated HRP is introduced. After a final wash to remove any unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of PYY bound in the initial step. The reaction is stopped, and the intensity of the color is measured.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Rat peptide YY (PYY) ELISA kit (7.8-500 pg/mL) is used to quantify PYY in serum, plasma, cell culture supernatant, and tissue homogenates of rats, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Tissue homogenates

Detection Range: 7.8-500 pg/mL

Sensitivity: 1.95 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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