Porcine proinsulin (PI) ELISA kit (1.56-100 pmol/L)

Cat. No.: 0126WXX-1281

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: PI

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Porcine proinsulin (PI) ELISA kit (1.56-100 pmol/L)

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Product Overview

Description: Porcine proinsulin (PI) ELISA kit (1.56-100 pmol/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PI in porcine samples with a range of 1.56-100 pmol/L and a minimum detectable dose (sensitivity) of 0.5 pmol/L. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a porcine PI monoclonal antibody, and a biotin-labeled polyclonal antibody is used for detection. Samples and the biotinylated antibody are added to the wells and washed with PBS or TBS. Avidin-peroxidase conjugates are then introduced. Following a thorough wash, TMB substrate is added for coloration. The blue catalytic product turns yellow under acidic conditions. The resulting color depth is positively correlated with the concentration of the target analyte in the sample.

Background: Proinsulin is the precursor molecule of insulin, synthesized in pancreatic β cells and subsequently processed into mature insulin and C-peptide. It plays a critical role in glucose homeostasis by serving as an essential intermediate in insulin biosynthesis and secretion. Under normal physiological conditions, proinsulin is efficiently converted to insulin; however, impaired processing or increased proinsulin secretion reflects β-cell dysfunction. Elevated circulating proinsulin levels are commonly observed in obesity and insulin resistance, making proinsulin a key indicator of metabolic stress and a contributing factor to the development of obesity-associated type 2 diabetes.

Synonyms: INS; Pro-Insulin; Preproinsulin

Applications: Porcine proinsulin (PI) ELISA kit (1.56-100 pmol/L) is used to quantify PI in serum, plasma, cell culture supernatant, organizations of porcine, providing data to support research in a wide range of areas, including endocrinology, hormone metabolism, obesity, and others.

Research Area: Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Porcine serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 1.56-100 pmol/L

Sensitivity: 0.5 pmol/L

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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