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Porcine glutathione peroxidase 1 (GPX1) ELISA kit-Quantitative sandwich
Cat. No.:
0126WXX-263
Assay Type:
Quantitative sandwich ELISA
Target Species:
Porcine
Assay Target:
GPX1
Size:
Add to basket
Product Overview
Description:
Porcine glutathione peroxidase 1 (GPX1) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GPX1 in porcine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.068 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The ELISA analytical biochemical technique is based on GPX1 antibody-GPX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX1 antigen targets in samples.
Background:
GPX1 is an antioxidant enzyme widely distributed in the cytoplasm, mitochondria, and nucleus. It exists as a homotetramer and primarily functions to protect cells and enzymes from oxidative damage. GPX1 maintains intracellular redox homeostasis by catalyzing the reduction of hydrogen peroxide and lipid peroxides. It plays a crucial role in multiple biological processes, including apoptosis, lipid metabolism, inflammatory responses, and the response to oxidative stress. Oxidative stress is considered one of the causes for the development of obesity-related metabolic disorders. GPx-1 is a potential regulator of obesity.
Synonyms:
GSHPX1; Cellular glutathione peroxidase
Applications:
Porcine glutathione peroxidase 1 (GPX1) ELISA kit is used to quantify GPX1 in tissue homogenates, cell lysates, and other biological fluids of porcine, providing data to support research in a wide range of areas, including endocrinology, inflammation, oxidative stress, metabolism, obesity, and others.
Research Area:
Endocrinology; Inflammation; Oxidative stress; Metabolism; Obesity
Specification
Sample Type:
Porcine tissue homogenates; Cell lysates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.068 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).



