Pigeon glutathione peroxidase 1 (GPX1) ELISA kit (12.5-800 U/mL)

Cat. No.: 0126WXX-262

Assay Type: Quantitative ELISA

Target Species: Pigeon

Assay Target: GPX1

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Pigeon glutathione peroxidase 1 (GPX1) ELISA kit (12.5-800 U/mL)

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Product Overview

Description: Pigeon glutathione peroxidase 1 (GPX1) ELISA kit (12.5-800 U/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GPX1 in pigeon samples with a range of 12.5-800 U/mL and a minimum detectable dose (sensitivity) of <4.69 U/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to the target antigen. Standards or samples are added to the wells along with a biotin-conjugated antibody preparation. Subsequently, avidin-HRP is added and incubated. Upon the addition of TMB substrate, a color change occurs only in wells containing the target antigen complex. The reaction is terminated with a sulphuric acid solution, and the absorbance is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of the target antigen is determined by comparing the O.D. of the samples to the standard curve.

Background: GPX1 is an antioxidant enzyme widely distributed in the cytoplasm, mitochondria, and nucleus. It exists as a homotetramer and primarily functions to protect cells and enzymes from oxidative damage. GPX1 maintains intracellular redox homeostasis by catalyzing the reduction of hydrogen peroxide and lipid peroxides. It plays a crucial role in multiple biological processes, including apoptosis, lipid metabolism, inflammatory responses, and the response to oxidative stress. Oxidative stress is considered one of the causes for the development of obesity-related metabolic disorders. GPx-1 is a potential regulator of obesity.

Synonyms: GSHPX1; Cellular glutathione peroxidase

Applications: Pigeon glutathione peroxidase 1 (GPX1) ELISA kit (12.5-800 U/mL) is used to quantify GPX1 in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids of pigeon, providing data to support research in a wide range of areas, including endocrinology, inflammation, oxidative stress, metabolism, obesity, and others.

Research Area: Endocrinology; Inflammation; Oxidative stress; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids

Detection Range: 12.5-800 U/mL

Sensitivity: <4.69 U/mL

Precision (Intra-assay): CV≤6.6%

Precision (Inter-assay): CV≤9.7%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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