Mouse tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit

Cat. No.: OB0525WXX-136

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: TRACP-5b

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Mouse tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit is a ready-to-use ELISA kit for analyzing mouse TRACP-5b levels with a detection range of 0.078-5 mIU/mL. Its sensitivity is less than 0.02 mIU/mL and is an efficient dynamic research tool.

Assay Principle: The assay employs a quantitative sandwich enzyme immunoassay technique. An antibody specific for TRACP-5b has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any TRACP-5b is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific to TRACP-5b is added to the wells. After washing, avidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Background: ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.

Synonyms: TRAP 5b; sTRAP (Serum tartrate-resistant acid phosphatase, used in a clinical context); Tartrate-resistant acid phosphatase type 5b

Applications: Mouse tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit is designed for the in vitro quantitative analysis of TRACP-5b levels in mouse serum, plasma, and tissue homogenate samples.

Research Area: Protein dephosphorylation; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 0.078-5 mIU/mL

Sensitivity: <0.02 mIU/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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