Mouse tartrate-resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.781-50 ng/mL)

Cat. No.: 0126WXX-1991

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: TRACP 5b

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Mouse tartrate-resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.781-50 ng/mL)

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Product Overview

Description: Mouse tartrate-resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.781-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRACP 5b in mouse samples with a range of 0.781-50 ng/mL and a minimum detectable dose (sensitivity) of 0.47 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The micro ELISA plate provided has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate wells to bind with the specific capture antibody. Subsequently, a biotinylated detection antibody specific for mouse TRACP-5b and an avidin-HRP conjugate are added successively and incubated. After washing away unbound components, the substrate reagent is introduced; wells containing the mouse TRACP-5b complex will turn blue. The reaction is terminated by the Stop Solution, resulting in a yellow color. The OD is measured at 450 nm ± 2 nm, and the OD value is proportional to the concentration of mouse TRACP-5b.

Background: TRACP 5B, a metallophosphoesterase located at 19p13.2, encodes a tartrate-resistant acid phosphatase (EC 3.1.3.2) essential for bone resorption and the negative regulation of inflammatory cytokines, including TNF and interleukin-1 beta. By dephosphorylating osteopontin and bone sialoprotein, it maintains skeletal integrity and immune homeostasis; consequently, inactivating mutations lead to SPENCDI, while its elevated expression serves as a biomarker for various leukemias and Gaucher disease. In the context of metabolic health, TRACP 5B is increasingly recognized for its role in obesity-driven chronic inflammation, as its regulation of osteopontin signaling and macrophage activity directly influences the adipose tissue remodeling and systemic insulin resistance associated with excessive weight gain.

Synonyms: ACP5; TRAP5b; SPENCDI; TRACP-5b

Applications: Mouse tartrate-resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.781-50 ng/mL) is used to quantify TRACP 5b in serum, plasma, and biological fluids of mice, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, and others.

Research Area: Signal transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.781-50 ng/mL

Sensitivity: 0.47 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 89-108%

Storage: Store at 2-8°C.

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