Mouse peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1486

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: PPARα

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Mouse peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Mouse peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in mouse samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a double-sandwich ELISA technique with a mouse PPARα monoclonal capture antibody and a biotin-labeled polyclonal detection antibody. Samples and detection antibodies are added to the wells and washed with PBS or TBS, followed by the addition of avidin-peroxidase conjugates. After a final wash, a TMB substrate is introduced for visualization. The blue catalytic product turns yellow upon acidification, with the color intensity being positively correlated with the level of PPARα in the sample.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Mouse peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is used to quantify PPARα in serum, plasma, cell culture supernatant, and tissues of mice, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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