Mouse pancreatic lipase (PL) ELISA kit (2.5-50 mU/mL)

Cat. No.: 0126WXX-1402

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: PL

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Mouse pancreatic lipase (PL) ELISA kit (2.5-50 mU/mL)

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Product Overview

Description: Mouse pancreatic lipase (PL) ELISA kit (2.5-50 mU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PL in mouse samples with a range of 2.5-50 mU/mL and a minimum detectable dose (sensitivity) of 0.1 mU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PL ELISA kit applies a competitive enzyme immunoassay technique utilizing a monoclonal anti-PL antibody and a PL-HRP conjugate. Samples and assay buffer are incubated with the PL-HRP conjugate in a pre-coated plate for one hour. After five wash cycles, an HRP substrate is added to form a blue complex, which shifts to yellow upon adding a stop solution. The intensity of the color is inversely proportional to the PL concentration, as sample PL competes with the PL-HRP conjugate for a limited number of antibody binding sites. A standard curve is used to interpolate the PL concentration for each sample.

Background: PL, a key member of the lipase gene family located at chromosomal position 10q26.1, is a specialized carboxyl esterase (EC 3.1.1.3) specifically expressed in the pancreas and secreted into the extracellular region. This hydrolase is essential for the efficient digestion of dietary fats, specifically driving the hydrolysis of insoluble, emulsified triglycerides into absorbable components. Beyond its primary role in lipid catabolism and cholesterol absorption, PNLIP is involved in retinoid metabolic processes and is the central molecular driver in Pancreatic Lipase Deficiency, making it a fundamental target for understanding gastrointestinal lipid digestion and its systemic impact on energy balance.

Synonyms: PNLIP; PTL; Lipase; pancreatic; Pancreatic triacylglycerol lipase

Applications: Mouse pancreatic lipase (PL) ELISA kit (2.5-50 mU/mL) is used to quantify PL in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of mouse, providing data to support research in a wide range of areas, including enzyme and kinase, metabolic pathway, Infection immunity, hepatology, obesity, and others.

Research Area: Enzyme & Kinase; Metabolic pathway; Infection immunity; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 2.5-50 mU/mL

Sensitivity: 0.1 mU/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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