Mouse hormone-sensitive lipase (HSL) ELISA kit (0.156-10 U/mL)

Cat. No.: 0126WXX-893

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: HSL

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Mouse hormone-sensitive lipase (HSL) ELISA kit (0.156-10 U/mL)

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Product Overview

Description: Mouse hormone-sensitive lipase (HSL) ELISA kit (0.156-10 U/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSL in mice samples with a range of 0.156-10 U/mL and a minimum detectable dose (sensitivity) of 0.039 U/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs a quantitative sandwich enzyme immunoassay technique. A capture antibody specific for HSL has been pre-immobilized onto a microplate. Standards and samples are pipetted into the wells, where any HSL present is bound by the stationary antibody. After removing unbound substances through washing, a biotin-conjugated detection antibody specific for HSL is added. Following a subsequent wash, avidin-conjugated HRP is introduced. After a final wash to remove any unbound reagents, a substrate solution is added, and color develops in proportion to the amount of HSL bound in the initial step. The reaction is stopped, and the signal intensity is measured.

Background: HSL is the key enzyme for fat breakdown. Belonging to the “GDXG” lipase family, this enzyme primarily hydrolyzes stored triacylglycerols into free fatty acids in adipose tissue and the heart. This process represents the rate-limiting step of lipolysis, controlling the overall speed of fat breakdown. HSL can be rapidly activated by catecholamine hormones (such as epinephrine) through a cyclic adenosine monophosphate (cAMP)-dependent phosphorylation process, while insulin regulates its dephosphorylation and inactivation. Due to its functional characteristics, HSL has become a central subject in obesity research, particularly in the regulation of fat storage and release.

Synonyms: LHS; LIPE; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Mouse hormone-sensitive lipase (HSL) ELISA kit (0.156-10 U/mL) is used to quantify HSL in serum, plasma, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including metabolic pathway, obesity, etc.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 0.156-10 U/mL

Sensitivity: 0.039 U/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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