Mouse fatty acid synthase (FAS) ELISA kit

Cat. No.: OB0625WXX-842

Assay Type: Quantitative competitive or sandwich ELISA

Target Species: Mouse

Assay Target: FAS

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Mouse fatty acid synthase (FAS) ELISA kit

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Product Overview

Description: Mouse fatty acid synthase (FAS) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in mouse with a specificity of 1.0 μg/mL.

Assay Principle: A microtiter plate is pre-coated with a monoclonal antibody specific for FAS. Standards or samples are then added to the wells of the microtiter plate, and FAS (if present) binds to the antibody-coated wells. To quantify the amount of FAS in the sample, a standard preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for FAS is added to each well to “clamp” the FAS immobilized on the microtiter plate. The microtiter plate is incubated, and the wells are then washed thoroughly to remove unbound components. A substrate solution is then added to each well. The enzyme (HRP) and substrate will react during the brief incubation period. Only wells containing FAS and enzyme-conjugated antibody will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of FAS in each sample is derived from this standard curve.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Mouse fatty acid synthase (FAS) ELISA kit is an important tool for the quantitative detection of FAS in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of mice.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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