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LipoKnoxa™ Human UCP2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX15
Species:
Human
Target Gene:
UCP2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
Add to basket
| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX15-1 | CTACTGCCACTGTGAAGTTTC | CDS | Inquiry |
| V0126XX15-2 | TTTCCTCTGGATACTGCTAAA | CDS | Inquiry |
| V0126XX15-3 | CCTCTTCCTTCCGCTCCTTTA | 3' UTR | Inquiry |
| V0126XX15-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human UCP2 shRNA Ad5 Particle (Silencing) is engineered to reduce the expression of UCP2, a key regulator of mitochondrial proton leak and reactive oxygen species (ROS) production in metabolic tissues. This product enables the investigation of UCP2’s influence on insulin secretion and energy efficiency. Supporting flexible promoter and reporter configurations, the system undergoes exhaustive quality control—including mycoplasma and sterility testing—to safeguard your research outcomes.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
UCP2
Full Name:
Uncoupling protein 2
Gene Symbol:
UCPH; BMIQ4; SLC25A8
Gene ID:
7351
RefSeq ID-1:
NP_001368872.1
RefSeq ID-2:
NM_001381943.1
Summary:
UCP2 is a crucial member of the mitochondrial uncoupling protein (UCP) family, functioning within the inner mitochondrial membrane. It mediates the "mitochondrial proton leak," a process that dissipates the proton gradient as heat, thereby uncoupling oxidative phosphorylation from ATP generation. Expressed broadly, particularly highly in skeletal muscle, UCP2 acts to reduce the mitochondrial membrane potential and may play a protective role against reactive oxygen species (ROS). Its systemic role in nonshivering thermogenesis and metabolic efficiency positions it as a significant candidate in research concerning the genetic basis of obesity and diabetes.
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