LipoKnoxa™ Human MC4R shRNA Ad5 Particle (Silencing)

Cat. No.: V0126XX3

Species: Human

Target Gene: MC4R

Vector System: Adenovirus

Modulation Type: Silencing (shRNA)

LipoKnoxa™ Human MC4R shRNA Ad5 Particle (Silencing)

INQUIRY Datasheet MSDS

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX3-1	CCATTGTCATCACCCTATTAA	CDS	Inquiry
V0126XX3-2	CCCATTCTTCCTCCACTTAAT	CDS	Inquiry
V0126XX3-3	AGCGGGTTGGGATCATCATAA	CDS	Inquiry
V0126XX3-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human MC4R shRNA Ad5 Particle (Silencing) enables the targeted knockdown of the MC4R, a critical regulator of energy intake and expenditure in the hypothalamus. Leveraging an advanced shRNA expression framework, this tool is essential for modeling monogenic obesity and exploring central satiety pathways. Our production process features stringent quality control, verifying functional potency and sterility to deliver consistent results in complex neuroendocrine experiments.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type 5 (dE1/E3)

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.

Sterility Test: This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.

Mycoplasma Test: This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.

Other QC: Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.

Storage: Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.

Stability: This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.

Shipping Condition: All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.

Handling Notes: Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.

Target Profile

Gene Name: MC4R

Full Name: Melanocortin receptor 4

Gene Symbol: BMIQ20

Gene ID: 4160

RefSeq ID-1: NP_005903.2

RefSeq ID-2: NM_005912.3

Summary: The protein encoded by the MC4R gene is a membrane-bound receptor and a member of the melanocortin receptor family. This encoded protein interacts via G-protein mediation with adrenocorticotropic hormone and melanocyte-stimulating hormones. Notably, this gene contains no introns. Defects in this gene can lead to autosomal dominant obesity.

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