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LipoKnoxa™ Human AKT2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX80
Species:
Human
Target Gene:
AKT2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX80-1 | CAAATGGACACGGCATCATAA | 3' UTR | Inquiry |
| V0126XX80-2 | AGTCTACCCTGGTGGTCATAA | 3' UTR | Inquiry |
| V0126XX80-3 | TGCGGAAGGAAGTCATCATTG | CDS | Inquiry |
| V0126XX80-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human AKT2 shRNA Ad5 Particle (Silencing) provides a specialized solution for silencing the AKT2 isoform, the primary mediator of insulin-induced glucose uptake in skeletal muscle and fat. This precise gene-knockdown tool is built on an advanced Ad5 platform with U6 or miR30-based shRNA systems to study the molecular drivers of Type 2 Diabetes. To ensure the integrity of your metabolic research, every batch undergoes total quality control screening for sequence accuracy and microbial contaminants.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
AKT2
Full Name:
AKT serine/threonine kinase 2
Gene Symbol:
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Gene ID:
208
RefSeq ID-1:
NP_001317440.1
RefSeq ID-2:
NM_001330511.1
Summary:
AKT2 encodes a serine/threonine kinase involved in multiple signaling pathways, including insulin signaling and cellular growth. Overexpression of AKT2 has been linked to oncogenic processes, while its normal function is essential for glucose uptake and energy homeostasis. Variants in AKT2 can contribute to insulin resistance and increased obesity risk.
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